Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) a IP using origin recognition complex-associated (ORCA) Ab from U2OS cells. ORCA, G9a, and Suv39H1 were analyzed by immunoblotting (IB). b IP using G9a Ab from U2OS cells. Endogenous ORCA, G9a, and Suv39H1 were analyzed by IB. ( B ) a and b Immunoprecipitation (IP) using ORCA antibody (Ab) from cells expressing T7-ORCA and different Flag-KMTs: a H3K9 KMTs G9a; b H3K9 KMT GLP and Suv39H1. ( C ) U2OS 2-6-3 CLTon cells co-transfected with individual YFP-LacI-KMTs and CFP-ORCA. Inset represents 150% magnification of the boxed region. ( D ) IP using T7 ab from cells co-expressing T7-ORCA and; a Flag-G9a or b Flag-Suv39H1 in the presence (+) or absence (−) of EtBr. ( E ) Direct interaction of ORCA and a G9a or b SUV39H1 using purified proteins. ‘*’ denotes cross reacting band and ‘ ’denotes ORCA. DOI: http://dx.doi.org/10.7554/eLife.06496.003

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: Western Blot, Immunoprecipitation, Expressing, Transfection, Purification

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) a. Endogenous ORCA IP in untransfected (UT) U2OS and in U2OS cells expressing full length (FL) or the SET domain of HA-Suv39H1. b. Endogenous ORCA IP in untransfected (UT) U2OS and in U2OS cells expressing full length (FL) or the Ankyrin (ANK) domain of HA-G9a. ( B ) IB showing efficient depletion of endogenous ORCA from U2OS nuclear extract by using ORCA antibody. ( C ) Flag IP in Hela cells stably expressing Flag-HA-G9a or Flag-HA-GLP. IPs were conducted using nuclear soluble (S) or chromatin (P) fractions and endogenous ORCA, ORC2, MCM3, Geminin and PCNA were analyzed by IB. ( D ) ORCA does not interact with arginine methyltransferase (RMT) PRMT5. IP using T7 Ab from cells expressing T7-ORCA and HA-PRMT5. DOI: http://dx.doi.org/10.7554/eLife.06496.004

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: Expressing, Stable Transfection

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) a Schematic representation of various truncation mutants of ORCA containing a T7-epitope on the N-terminus. The specific domains that can associate with G9a and Suv39H1 based on–IB is depicted as ‘+’. b Schematic representation of various truncation mutants of G9a containing a HA-epitope on the N-terminus. The interaction domain of G9a that interacts with ORCA is denoted as ‘+’. c Schematic representation of various truncation mutants of Suv39H1 containing a HA-epitope on the N-terminus. The interaction domain of Suv39H1 that interacts with ORCA is denoted as ‘+’. ( B ) a IP in U2OS cells expressing various HA-G9a mutants and T7-G9a using T7 Ab and analysis by T7 and HA-IB. b IP in U2OS cells expressing various HA-Suv39H1 mutants and T7-G9a using T7 Ab and analysis by T7 and HA IB. ‘*’ denotes the cross-reacting band. ( C ) a Cells co-transfected with YFP-LacI (negative control) and CFP-G9a or YFP-LacI-ORCA or the truncation mutants along with CFP G9a in CLTon cells. b The % of cells with CFP-G9a recruited to the locus is plotted. ( D ) a Cells co-transfected with YFP-LacI (negative control) and CFP-ORCA or YFP-LacI-G9a wild type and the mutants, which are catalytically inactive along with CFP-ORCA in CLTon cells. b The % of cells with CFP-ORCA recruited to the locus. ( E ) a U2OS 2-6-3 CLTon cells co-transfected with YFP-LacI-ORCA and CFP-G9a in the presence and absence of doxycycline. b The % of cells with CFP-G9a recruited to the locus in both conditions. Scale bars equal 10 μm. Inset represents 150% magnification of the boxed region. Error bars represent s.d., n = 3. ****p < 0.0001. DOI: http://dx.doi.org/10.7554/eLife.06496.005

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: Expressing, Transfection, Negative Control

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) a. IP in U2OS cells expressing various T7-ORCA mutants and FLAG-G9a using T7 Ab and analysis of G9a by IB. b. IP in U2OS cells expressing various T7-ORCA mutants and HA-Suv39H1 using T7 Ab and analysis of Suv39H1 by IB. ( B ) a. Cells were co-transfected CFP-LacI and YFP-G9a (negative control) or CFP-LacI-ORCA along with YFP-G9a truncation mutants. b. The % of cells with YFP-G9a truncation mutants recruited to the locus is plotted. Note the significant reduction in the recruitment of YFP-G9a (aa1-965), the mutant lacking the SET domain, to the locus. Error bars represent s.d, n=3. ****p<0.0001. span=""> <0.0001. span="">( C ) Localization of H3K9me2 in CLTon cells in cells with (+) or without (-) the expression of YFP-LacI-G9a. Note the H3K9me2 accumulation in YFP-LacI-G9a expressing cells. ( D ) IP in U2OS cells expressing T7-ORCA and GFP-G9a-full length and ∆SET mutant using T7 Ab and analysis of GFP-G9a by IB. ( E ) Localization in CLTon cells expressing CFP-LacI-ORCA, of HP1α and YFP-CDK9, at heterochromatic (-Dox) as well as decondensed locus (+Dox). the expression of YFP-LacI-G9a. Note the loss of HP1α and accumulation of YFP-CDK9 upon decondensation of the locus. Scale bar, 10µm. Inset represents 150% magnification of the boxed region. ( F ) Tethering of YFP-LacI-Orc3 recruits Orc2 at heterochromatic (-Dox) as well as decondensed locus (+Dox). Scale bar, 10µm. Inset represents 150% magnification of the boxed region. DOI: http://dx.doi.org/10.7554/eLife.06496.006

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: Expressing, Transfection, Negative Control, Mutagenesis

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) a Representative single-molecule fluorescence time trajectories for YFP-tagged molecules that exhibit one-step, two-step, and three-step photobleaching. b Key to the schematics of the SiMPull assay. ( B ) a and b Schematic and total internal reflection fluorescence (TIRF) images of YFP molecules pulled down from U2OS cell lysates expressing T7-ORCA and YFP-ORC1 using biotinylated T7 Ab. The same lysate incubated with biotinylated HA Ab served as the control. c Average number of YFP fluorescent molecules per imaging area (5000 μm 2 ). d Photobleaching step distribution for YFP-ORC1 bound to T7-ORCA. Note 1:1 ratio of ORCA to Orc1. e Intensity profiles of the YFP-ORC1 molecules bound to T7-ORCA. ( C ) a – d ORCA-G9a pulldown. Shown are YFP molecules pulled down from U2OS cell lysates expressing T7-ORCA and YFP-G9a. Note 1:1 or 1:2 ratio of ORCA to G9a. e Intensity profiles of YFP-G9a molecules bound to T7-ORCA. ( D ) a – d ORCA-Suv39H1 pulldown. Shown are YFP molecules pulled down from U2OS cell lysates expressing T7-ORCA and YFP-Suv39H1. Note 1:1 ratio of ORCA to Suv39H1. e Intensity profiles of YFP-Suv39H1 molecules bound to T7-ORCA. ( E ) a – c Determination of ORCA complexes containing both ORC and G9a by SiMPull and colocalization analyses. a Schematic of YFP and mCherry molecules pulled down from U2OS cell lysates expressing T7-ORCA, YFP-ORC1, and mCherry-G9a using biotinylated T7 Ab. The same lysate incubated with biotinylated HA Ab served as the control. b Average number of YFP and mCherry fluorescent molecules per imaging area (5000 μm 2 ). c Note 39 ± 5% overlap. Transfection condition used as indicated in , lane3. ( F ) a – c Determination of ORCA complexes containing multiple H3K9 KMTs by SiMPull and colocalization analyses. a Schematic of YFP and mCherry molecules pulled down from U2OS cell lysates expressing T7-ORCA, YFP-Suv39H1, and mCherry-G9a using biotinylated T7 Ab. The same lysate incubated with biotinylated HA Ab served as the control. b Average number of YFP and mCherry fluorescent molecules per imaging area (5000 μm 2 ). c Note 55 ± 7% colocalization. Transfection condition used as indicated in , lane3. Scale bars, 10 μm. Error bars represent s.d., n = 3. DOI: http://dx.doi.org/10.7554/eLife.06496.007

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: Fluorescence, Expressing, Incubation, Control, Imaging, Transfection

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) a.Titration of T7-ORCA, mCherry-G9a and YFP-ORC1 plasmids in U2OS cells. b-c. Determination of ORCA complexes containing both ORC and G9a by SiMPull and colocalization analyses. b. Average number of YFP and mCherry fluorescent molecules per imaging area (5000µm 2 ). c. Note 41±4% overlap. Transfection condition used as indicated in Fig. S3Aa, lane 5. ( B ) a.Titration of T7-ORCA, mCherry-G9a and YFP-Suv39H1 plasmids in U2OS cells. b-c. Determination of ORCA complexes containing both G9a and Suv39H1 by SiMPull and colocalization analyses. b. Average number of YFP and mCherry fluorescent molecules per imaging area (5000µm 2 ). c. Note 46±11% overlap. Transfection condition used as indicated in Fig. S3Ba, lane 5. ( C ) Sequential IP of HA-Orc1 followed by T7-ORCA from U2OS extracts expressing T7-ORCA, HA-Orc1 and Flag-G9a. IB of G9a corroborated the presence of Orc1-ORCA-G9a triple complex. ( D ) Sequential IP of HA-G9a followed by T7-ORCA from U2OS extracts expressing T7-ORCA, HA-G9a and Flag-Suv39H1. IB of Suv39H1 corroborated the presence of G9a-ORCA-Suv39H1 triple complex. DOI: http://dx.doi.org/10.7554/eLife.06496.008

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: Titration, Imaging, Transfection, Expressing

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) a Schematic of experimental setup for peptide pulldown and analyses by SiMPull. b TIRF images of YFP-ORCA WT and 1–127 aa pulled down by H3K9 modified peptides. Note that the YFP-ORCA WT and 1–127 aa truncation mutant expressing lysates were diluted so that the concentration of the overexpressed proteins is comparable (200 and 800 times, respectively for WT and 1–127 aa). c Average number of fluorescent molecules per imaging area. Scale bars, 10 μm. ( B ) a Chromatin fractionation in ORCA-depleted U2OS cells followed by IB analysis of H3K9me2 and me3. b Chromatin fractionation in ORCA-depleted diploid fibroblasts, WI38 followed by IB analysis of H3K9me2 and me3. Splicing factor, SRSF1 is shown as a loading control. Error bars represent s.d., n = 3. S and S2-cytosolic; S3-nuclear soluble and MNase sensitive; P: nuclear; P3: nuclear insoluble and MNase resistant fraction. DOI: http://dx.doi.org/10.7554/eLife.06496.009

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: Modification, Mutagenesis, Expressing, Concentration Assay, Imaging, Fractionation, Control

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) Model-based analysis of ChIP-sequencing (MACS) 1.4 peaks analysis of H3K9me3 ChIP-seq in control and ORCA-depleted cells. ( B ) Regions showing greater than fivefold decrease in H3K9me3 upon ORCA knockdown (KD) plotted along the length of the chromosomes in which they reside. ( C ) a Normalized number of reads of repetitive sequences in control and ORCA KD H3K9me3 ChIP-seq. Normalized number of reads of- b Telomeric repetitive sequences and c Centromeric repetitive sequences in control and ORCA KD H3K9me3 ChIP-seq. ( D ) a – d Representative regions showing significant decrease in reads in H3K9me3 ChIP on ORCA KD compared to the control. ( E ) a HA-ORCA ChIP at H3K9me3-target sites and ( b ) C-FOS. ( F ) a Suv39H1 ChIP and b IgG ChIP at regions showing decrease in H3K9me3. Error bars represent s.d., n = 3. C-FOS is shown as negative control. DOI: http://dx.doi.org/10.7554/eLife.06496.011

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: ChIP-sequencing, Control, Knockdown, Negative Control

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) a. Regions with H3K9me3 peaks detected by ChIP-seq plotted along the length of the chromosomes in which they reside. Chromosome scale indicated at the bottom of the chromosomes. b. Regions showing less than 1.3 fold decreases in H3K9me3 upon ORCA knockdown plotted along the length of the chromosomes in which they reside. Chromosome scale is indicated at the bottom of the chromosomes. ( B ) a-c. Representative regions showing significant decrease in the reads in H3K9me3 ChIP on ORCA knockdown. c-FOS, a region which doesn’t show decrease in H3K9me3 is also shown. ( C ) a-b. H3K9me2 ChIP at regions showing decrease in H3K9me3. ( D ) Chromatin fractionation in ORCA depleted U2OS cells and G9a and Suv39H1 IB analyses. SRSF1, a splicing factor, was used as loading control. ( E ) a. G9a ChIP and b. IgG ChIP at regions showing alterations in H3K9me2 and me3. Error bars represent s.d, n=3. DOI: http://dx.doi.org/10.7554/eLife.06496.012

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: ChIP-sequencing, Knockdown, Fractionation, Control

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) a – b HA-IP in control and ORCA-depleted U2OS cells co-expressing with HA-G9a and Flag-Suv39H1. ( B ) a TIRF images of GFP SiMPull in control and ORCA-depleted U2OS cells co-transfected with YFP-Suv39H1 and mCherry-G9a. The same lysates incubated with biotinylated HA Ab served as the control. b Average number of YFP fluorescent molecules per imaging area (5000 μm 2 ). c The % of mCherry-G9a pulled down by YFP Suv39H1 in control and ORCA KD. ( C ) a TIRF images of GFP SiMPull in U2OS cells transiently transfected with YFP-Suv39H1, mCherry-G9a, and T7-ORCA full-length or truncation mutant 1–270 or 128–647. The same lysates incubated with biotinylated HA Ab served as the control. b Average number of YFP fluorescent molecules per imaging area (5000 μm 2 ). c The % of mCherry-G9a pulled down by YFP-Suv39H1. The % of mCherry-G9a pulled down by YFP-Suv39H1 in WT-ORCA is 25 ± 1%; 1–270 ORCA is 14 ± 3%; and 128–647 ORCA is 29 ± 6%. Scale bars, 20 μm. Error bars represent s.d., n = 3. **p < 0.01, ***p < 0.001. DOI: http://dx.doi.org/10.7554/eLife.06496.013

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: Control, Expressing, Transfection, Incubation, Imaging, Mutagenesis

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) IB showing efficient siRNA-mediated KD of ORCA. ( B ) Distribution of S-phase cells displaying early, mid, and late replication patterns in control and ORCA KD cells. Error bars represent s.d., n = 3 independent experiments with 500 BrdU positive cells scored in each. ( C ) BrdU-PI flow cytometry of control and ORCA KD cells. ( D ) Replication timing of genomic regions that show reduced H3K9me3 upon ORCA KD. Gray bars represent late-replicating domains, and black bars denote early replicating domains. HeLa-S3 G1b and HeLa-S3 S1 are late G1 and early S cell cycle fractions that together represent the early replicating regions of the genome. DOI: http://dx.doi.org/10.7554/eLife.06496.014

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: Control, Flow Cytometry

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) Patterns of BrdU incorporation in S phase. Examples of early (1), mid (2 and 3) and late (4 and 5) S patterns. Scale bar, 10µm. ( B ) Replication timing of genomic regions that show reduced H3K9me3 upon ORCA knockdown. Gray bars represent late replicating domains and black bars denote early replicating domains. ( C ) BrdU ChIP in S phase in control and ORCA knockdown cells. Note the changes in replication timing of CELSR3 (b) and FAM20A (c) upon loss of ORCA. C-FOS locus is used as a control region whose replication timing remains unaffected upon loss of ORCA (a). Fold enrichment in the graph represents the % input of BrdU ChIP over % input of rIgG ChIP. DOI: http://dx.doi.org/10.7554/eLife.06496.015

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: BrdU Incorporation Assay, Knockdown, Control

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) Schematic of depletion of ORCA using the proteotuner system. ( B ) Western blotting showing the levels of endogenous and exogenous ORCA in the presence of control and ORCA siRNA. β′′, a nuclear speckle protein, serves as the loading control. Note that the DD-T7-ORCA-siRNA non-targetable version (NTV) is stabilized upon the addition of Shield1. ( C ) Chromatin fractionation and IB showing the levels of chromatin bound Orc2 in control and ORCA siRNA-treated cells (either in the absence or presence of exogenous ORCA). Note the reduction in chromatin bound Orc2 in the absence of ORCA and the rescue of its levels upon expression of exogenous ORCA. Also note the increase in the soluble pool of Orc2 in the absence of ORCA and the decrease of its levels upon expression of exogenous ORCA. Splicing factor, SRSF1 is shown as a loading control. ( D ) IB showing the levels of endogenous and exogenous ORCA at G1/S and 12 hr post-release from aphidicolin. H3 is used as loading control. ( E ) a – b Patterns of BrdU incorporation in control and ORCA-depleted cells in late S-phase. The white arrowheads indicate preferential incorporation of BrdU incorporation at perinucleolar regions upon loss of ORCA. Scale bar, 10 μm. b % increase in S-phase cells displaying early and % decrease of the mid and late replication patterns in ORCA-depleted cells compared to control cells. Error bars represent s.d., n = 3 independent experiments with ∼450 BrdU positive cells scored in each. ( F ) a H3K9me3 and HP1α immunofluorescence in control and ORCA-depleted cells. The white arrowheads indicate H3K9me3 and HP1α immunofluorescence at perinucleolar regions upon loss of ORCA. Representative regions in control and ORCA-depleted cells marked by white dotted squares (1, 2, and 3) are shown at 3× magnification on the right. Scale bar, 10 μm. b The % of cells with HP1α at nucleolar periphery in control and ORCA-depleted cells. Error bars represent s.d., n = 3. DOI: http://dx.doi.org/10.7554/eLife.06496.016

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: Western Blot, Control, Fractionation, Expressing, BrdU Incorporation Assay, Immunofluorescence

Journal: eLife

Article Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

doi: 10.7554/eLife.06496

Figure Lengend Snippet: ( A ) IP of DD-T7-ORCA siRNA NTV from U2OS cells using T7 Ab. DD-T7-ORCA siRNA NTV and endogenous Orc were analyzed by IB. ( B ) Patterns of BrdU incorporation in control and ORCA depleted cells in late S phase. Scale bar, 10µm. ( C ) H3K9me3 and HP1α immunofluorescence in control and ORCA depleted cells. Scale bar, 10µm. ( D ) Flow cytometry of control and ORCA knockdown cells at 0, 4, 8 and 12h post release from Aphidicolin block. DOI: http://dx.doi.org/10.7554/eLife.06496.017

Article Snippet: The following dataset was generated: Sumanprava Giri , Vasudha Aggarwal , Julien Pontis , Zhen Shen , Arindam Chakraborty , Abid Khan , Craig Mizzen , Kannanganattu V Prasanth , Slimane Ait-Si-Ali , Taekjip Ha , Supriya G Prasanth , 2015 , U2OS H3K9me3 ChIPseq , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68129 , Publicly available at NCBI Gene Expression Omnibus (GSE68129).

Techniques: BrdU Incorporation Assay, Control, Immunofluorescence, Flow Cytometry, Knockdown, Blocking Assay

Journal: Cell reports

Article Title: Transcription restores DNA repair to heterochromatin, determining regional mutation rates in cancer genomes

doi: 10.1016/j.celrep.2014.10.031

Figure Lengend Snippet: The x-axis of each graph shows increasing ChIP intensity of the heterochromatin-associated histone mark H3K9me3 (ENCODE data, Broad Institute, Panels A, C) and increasing inverse median RepliSeq values representing later replication time (ENCODE data, University of Washington, Panels B, D). The y-axis represents the mutation density per Kb divided by the individual mean. Plotted are values for either 8 aggregated repair wild-type (WT) cancers (solid blue line) or 5 aggregated XPC−/− cancers (broken orange line) for 8 equally sized genomic bins covering approximately 2Gb of expressed genome and 1Gb of non-expressed genome (+/− STD). Whereas mutation density correlates positively with increasing H3K9me3 and later replication time for expressed regions in repair wild-type cancers, these associations are diminished in XPC−/− samples (Panels A, B). In non-expressed portions of the genome, regional disparities in mutation density are almost completely abolished in XPC−/− samples (Panels C, D), indicating loss in the absence of GG-NER. See Figure S1 for additional data with sparser active marks H3K27ac and H3K4me1 and Table S1 for additional information on tumor samples.

Article Snippet: Increased mutation density was also associated with sparser active histone marks such as H3K27ac and H3K4me1, and these relationships were once again absent within non-expressed regions of XPC −/− cancers ( Figure S1 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 caption a8 Regional disparities in mutation density are absent in non-expressed portions of the genome of germline XPC −/− squamous cell carcinomas The x -axis of each graph shows increasing ChIP intensity of the heterochromatin-associated histone mark H3K9me3 (ENCODE data, Broad Institute, Panels A, C) and increasing inverse median RepliSeq values representing later replication time (ENCODE data, University of Washington, Panels B, D).

Techniques: Mutagenesis

Journal: Cell reports

Article Title: Transcription restores DNA repair to heterochromatin, determining regional mutation rates in cancer genomes

doi: 10.1016/j.celrep.2014.10.031

Figure Lengend Snippet: The x-axis denotes increasing expression in NHEK, measured in RPKM (plotted on a log scale). On the y-axis is the mutation density per Kb. Values are plotted for three independent WT cSCCs (A–C) and three independent XPC−/− cSCCs (D–F). The plots show six different H3K9me3 densities representing different chromatin levels, represented by distinct colors, for the transcribed (solid line) and untranscribed (broken line) strands. In WT cancers, both strands show decreasing mutation density in tightly-packaged DNA, illustrating robust domain-associated repair (DAR). DAR restores mutation rate in the most heterochromatic genomic regions to that of euchromatic regions, evidencing a dominant effect over chromatin state, but negligible additional impact in euchromatin (low H3K9me3). Even lower mutation density is seen from lesions on the transcribed strand, presumably representing TC-NER. In contrast, the XPC/− cancers show an absence of DAR, represented by an absence of transcription-dependent repair on the untranscribed strand, but intact TC-NER. See Figure S2 for additional samples and Table S2–S6 for more detailed mutation density information.

Article Snippet: Increased mutation density was also associated with sparser active histone marks such as H3K27ac and H3K4me1, and these relationships were once again absent within non-expressed regions of XPC −/− cancers ( Figure S1 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 caption a8 Regional disparities in mutation density are absent in non-expressed portions of the genome of germline XPC −/− squamous cell carcinomas The x -axis of each graph shows increasing ChIP intensity of the heterochromatin-associated histone mark H3K9me3 (ENCODE data, Broad Institute, Panels A, C) and increasing inverse median RepliSeq values representing later replication time (ENCODE data, University of Washington, Panels B, D).

Techniques: Expressing, Mutagenesis

Journal: Cell reports

Article Title: Transcription restores DNA repair to heterochromatin, determining regional mutation rates in cancer genomes

doi: 10.1016/j.celrep.2014.10.031

Figure Lengend Snippet: The x-axis shows increasing H3K9me3 intensity, representing a more repressive chromatin state. The y-axis shows the fold increase of the probability of a mutation, given a 50% decrease in expression level, referred to here as θ. Plotted is θ for 20,841 1Kb segments covering transcribed portions of 261 genes recently identified as recurrently mutated in human cancers. Highlighted are 1Kb fragments containing exons for the SCC tumor suppressors TP53 (A), NOTCH1 (B), IRF6 (C), as well as for the gene with exons of greatest average level of such mutation variance, CDC27 (D), which has been shown to be mutated at about 4% in melanomas and 2% in head and neck SCCs. Exons with the highest variance and its corresponding θ are indicated. See Table S7 for θ for all 20,841 1Kb segments.

Article Snippet: Increased mutation density was also associated with sparser active histone marks such as H3K27ac and H3K4me1, and these relationships were once again absent within non-expressed regions of XPC −/− cancers ( Figure S1 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 caption a8 Regional disparities in mutation density are absent in non-expressed portions of the genome of germline XPC −/− squamous cell carcinomas The x -axis of each graph shows increasing ChIP intensity of the heterochromatin-associated histone mark H3K9me3 (ENCODE data, Broad Institute, Panels A, C) and increasing inverse median RepliSeq values representing later replication time (ENCODE data, University of Washington, Panels B, D).

Techniques: Mutagenesis, Expressing